GC content of the plasmids used for the generation of the mutagenesis libraries. For each nucleotide position in the plasmid sequence, GC content was calculated using a 25 bp sliding window. The mutated sequences are highlighted in red. Intra-molecular ligation is very efficient and chances of the formation of the https://sitedirectedmutagenesis87531.thenerdsblog.com/27290932/die-besten-side-of-mutagenesis